With this experimental design, we aim to provide ... A junction PCR was designed with primers binding at each end of the loxP/lox2272. Clones with a band corresponding to the expected DNA size were ...

Partial E2 and E6 genes of HPV35 were amplified from DNA extracted from the cervical cancer tissue by primers 7 (F, R ... of HPV Genome in Cervical Cancer Tissue by Long-Range PCR and qPCR Currently, ...

Collectively, these findings provide insights into the rational design of HSV-vectored ... in Vero cells. RT-qPCR was performed as described in our previous study (Zhao et al., 2022). Data were ...

BEIJING, May 15, 2025 /PRNewswire/ -- Tsingke Biotech proudly participated in the AACR Annual Meeting 2025, held this April in Chicago, USA. As one of the world's premier events in oncology research, ...

This valuable study focuses on defining how the HSP70 chaperone system utilizes J-domain proteins to regulate the heat shock response-associated transcription factor HSF1. Using a combination of ...

PCR-Machines are based on Polymerase Chain Reaction (PCR) and produce copies of genes exponentially. The PCR cycling reactions are carried out in an automated thermocycler, which is capable of ...

Introducing the SM 50 from Retsch - a cutting mill that revolutionizes the benchtop market with unmatched performance and ...

8 Validated human primers are listed in online supplementary table 2. Reactions were run in duplicate for each gene on a ViiA7 qPCR machine (Applied Biosystems). Results were calculated using the DDCt ...

qPCR primers used in the experiment; single-cell transcriptome sequencing information on blood samples; gene expression of cell clusters; marker gene of erythrocyte subtype; pseudotime and KEGG ...

In this study, we developed a qPCR assay using specific primers and probes designed for the conserved sequences of six BRDC-associated pathogens. We optimized the reaction system and amplification ...