The yeast two-hybrid system is one of the most widely used functional genomic tools for studying protein-protein interactions. But transcriptional activators cannot be used as 'bait' proteins in the ...

Method: We employed yeast two-hybrid technology to search for interacting proteins of L5, combined with laser confocal microscopy to observe the subcellular localization of these candidate proteins, ...

Two-hybrid screening can be carried out as a benchtop assay, a high-throughput screen, or a library screen. The two-hybrid system has the advantage that it is an in vivo technique, meaning it is ...

Two characterized, phosphorylation‐dependent PPIs with unknown kinase–substrate relationships were analyzed in a phospho‐yeast two‐hybrid assay. CK2α1 and SGK2 kinases can abrogate the interaction ...

Two characterized, phosphorylation‐dependent PPIs with unknown kinase–substrate relationships were analyzed in a phospho‐yeast two‐hybrid assay. CK2α1 and SGK2 kinases can abrogate the interaction ...

A generally applicable two-hybrid assay demonstrates that MHC class I heavy chains devoid of beta-2 microglobulin associate ... Such challenges are not usually met by yeast two-hybrid screens and ...

A separate class of assays detects PPIs by signal integration rather than real-time imaging, and produces gene transcription as the readout. Examples include the yeast two hybrid assay (Miller and ...

The two-hybrid technique is a well-established cell-based method for analyzing protein–protein interactions. Originally developed for use in yeast, the system was later adapted for mammalian cells.

Bacterial Two-Hybrid Assay. The protein–protein interaction was detected by Bacterial Adenylate Cyclase Two-Hybrid System Kit (Euromedex) according to the product manuals. Libraries were tested ...

The yeast two-hybrid (Y2H) system exploits host cell genetics in order to display binary protein–protein interactions (PPIs) via defined and selectable phenotypes. Numerous improvements have been made ...