Our laboratory uses a quantitative fluorescence microscopy assay to measure the exocytosis of lysosomes towards agLDL during digestive exophagy. (23,27) In this experiment, we deliver ...

In macrophages, triggering of pyropotosis by the SUMO E3 ligase MAPL causes transfer of mitochondrial DNA (mtDNA) to lysosomes via VPS35. Subsequently, activated gasdermin D and E can be recruited to ...

This study defines a homeostatic mechanism that allows macrophages and microglia to match the degradative activity of their lysosomes to ongoing changes in cellular demand. It shows that the ...

The role of macrophages in the immune system and LSDs was not well known, but the new research published in Nature Cell Biology in an article titled, “Pressure sensing of lysosomes enables ...

When functional, a lysosome can break down large sugars into small sugars, which are then used as an energy source. In LSDs, these macrophage lysosomes become swollen and filled with waste.

In this image, cells (blue) are stressed due to the build-up and storage of lysosomes (yellow). The Hospital for Sick Children (SickKids) New research indicates that inflammation in macrophages caused ...

Cystic fibrosis (CF) human and mouse macrophages are defective in their ability to clear bacteria such as Burkholderia cenocepacia. The autophagy process in CF (F508del) macrophages is halted, and the ...

The fused lysosome and phagosome is referred to as a phagolysosome. Most macrophages can live for several months and can kill hundreds of different bacteria before they die. In this way ...

Understanding the derangements in cellular processes that underlie macrophage dysfunction is critical to the development of future therapeutics. The autophagy-lysosome system in macrophages plays ...

in lysosomes. Thus, engulfed apoptotic cells can be clearly identified by their strong red fluorescence. We incubated the pHrodo Red-labeled apoptotic thymocytes for 2 hr with sorted thymic or ...