Plasmid DNA (pDNA) constructs are used for a number of key applications, including gene therapy and mRNA. Manufacturing ...

An obscure group of phages offers insights into elements of microbial warfare—and blueprints for new antibiotics.

This valuable study presents a novel approach to enhance the therapeutic potential of mesenchymal stromal cells (MSCs) by genetically modifying their glycogen synthesis pathway, resulting in increased ...

Lentiviral infection. HEK293T cells were seeded (2.5x10 6) on 10 cm plate coated with poly-L ... 600 µL of Opti-MEM (Gibco), 22 µL of Mirus LT1 transfection reagent (Mirus) and 4 µg of lentiviral ...

Technologies used for cell and gene therapy development have come a long way but there are still important challenges associated with lentivirus manufacture. GMP plasmids account for a substantial ...

This enables lentiviral production from a single-plasmid transfection, in contrast to the standard four-plasmid process. In lentiviral manufacturing, GMP plasmids account for a substantial ...

WEST JEFFERSON, OH and GAITHERSBURG, MD / ACCESSWIRE / October 31, 2023 / AmplifyBio, a leading service provider for the commercialization of advanced modality therapeutics, and Vector BioMed, a ...

Charles River Laboratories International, Inc. (NYSE: CRL) today announced the launch of its off-the-shelf lentiviral vector (LVV) packaging plasmids, providing a streamlined solution to the ...

New plasmids will help accelerate and standardize lentiviral vector development from clinical to commercial manufacturing. WILMINGTON, Mass.--(BUSINESS WIRE)--Charles River Laboratories International, ...

Lentiviral vectors dominate the manufacturing of the CAR-T cells. The production of the lentiviruses is typically based on transient transfection where 4 GMP-grade bacterial plasmids have to be ...