We used a yeast two-hybrid approach: LexA-BD-fused A2IC protein was the bait for screening a human monocyte cDNA library in which GAL4-AD fusion proteins were the prey.

Schematic illustration of the LexA-based yeast two-hybrid interaction. Source: OriGene Technologies, Inc. 97/98 Catalog. Reprinted with permission. The DupLEX-ATM system from OriGene Technologies is a ...

Thus, a two-hybrid screen can be performed with two different baits at the same time, with each bait activating individual reporter genes. Figure 1: Differential yeast strain HW18.