For immunoblots, total ALK was detected using monoclonal ALK1 (anti-human CD246, DakoCytomation) or polyclonal (Zymed) antibodies. Y1586 phosphorylated ALK was revealed using the number 3343 ...

Equal parts of the remaining supernatants were then exposed to rabbit anti-Numb (Upstate), goat anti-Numb (Novus Biologicals), rabbit anti-Grin1 (Chemicon), mouse anti-Cdh1 (Zymed), mouse anti ...