To test RNA-RNA interactions in cells, we developed a yeast RNA hybrid system derived from the yeast three-hybrid system. In this setup, the activation of a reporter gene (HIS3 or lacZ) is ...

The three major variations of the yeast two-hybrid system are described in Part I by those who were responsible for their creation. The major differences between the variations include the DNA ...

Some of these include the mammalian two-hybrid system for use with mammalian host cells, and the yeast three-hybrid systems. The three-hybrid, or tribrid, system employs dimeric small molecule ...

Then, in the late-1980s, a new option emerged: the yeast two-hybrid (Y2H) assay. The brainchild of Stanley Fields, a Howard Hughes Medical Institute investigator at the University of Washington, Y2H ...

The DUALmembrane yeast two-hybrid technology invented and patented by Dualsystems, based in Schlieren (Switzerland), has been especially designed to screen for membrane protein interactions. It is ...

The ultimate realization of the yeast two-hybrid (Y2H) assay is the interactome. In theory, an interactome is a proteome-wide map of a cell's protein-protein interactions. In practice, they represent ...