Using the new system, sample preparation of 96 CAGE cDNA libraries, which would normally take a manual operator 42 days, is done in only 8 days. The researchers further demonstrated, through a ...

Reverse transcription-specific target amplification (RT-STA) master mix preparation ... is capable of synthesizing specific cDNA from a total RNA sample. The second enzyme in the master mix ...

Preparation of cDNA ... Prep IFC using the SMARTer PCR cDNA Synthesis kit (Clontech) and the Advantage 2 PCR kit (Clontech). 1 μl of the ERCC Spike-In Control Mix (Ambion) in a 1:400 dilution ...

Using the new system, sample preparation of 96 CAGE cDNA libraries, which would normally take a manual operator 42 days, is done in only 8 days. The researchers further demonstrated, through a ...